Journal: Scientific Reports

Article Title: Hypoionic shock treatment enables aminoglycosides antibiotics to eradicate bacterial persisters

doi: 10.1038/srep14247

Figure Lengend Snippet: ( a ) Survival ratio of stationary phase MC4100 (streptomycin-resistant) or BW25113 (streptomycin-sensitive) E. coli cells after being treated with tobramycin- (500 μg/ml) or streptomycin-containing (2 mg/ml) distilled water. Asterisks indicate no detection of survival. ( b ) Time-dependent fluorescence-activated cell sorting analysis (left) and direct fluorescent microscopy imaging (right; at the 30 minutes point) of stationary phase cells that were treated with tobramycin-containing (500 μg/ml) distilled water before incubated with propidium iodide (50 μg/ml) in PBS or LB for the indicated length of time. A.U., arbitrary units. ( c ) Regular micrographs of the cells taken at the indicated time point. Scale bar, 5 μm.

Article Snippet: Stationary phase Gram-negative Escherichia coli bacteria cells of strains BW25113 and MC4100 or Gram-positive Staphylococcus aureus bacteria cells of the strain ATCC 25923 were prepared by overnight culturing frozen stock bacteria cells at 37 °C, 200 r.p.m. in 20 ml of Luria-Bertani (LB) growth medium.

Techniques: Fluorescence, FACS, Microscopy, Imaging, Incubation

Journal: Applied and Environmental Microbiology

Article Title: Disulfide Bond Formation and Activation of Escherichia coli ?-Galactosidase under Oxidizing Conditions

doi: 10.1128/AEM.06923-11

Figure Lengend Snippet: Specific biological activity (measured by either absorbance or fluorescence). The specific activities of rβ-galactosidase (A) and rGFP (B) produced in both the wild-type (wt [MC4100]) and Origami E. coli strains were determined. The absolute values for biological activity are given above the bars. The P values, obtained by a t test, are 0.184 (A) and 0.060 (B).

Article Snippet: The Escherichia coli strains used in this work were the K-12 derivatives MC4100 [ araD139 Δ( argF-lac ) U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR Str r ] ( 43 ), Origami 2 {Δ( ara-leu ) 7697 Δ lacX74 Δ phoA PvuII phoR araD139 ahpC galE galK rpsL F′[ lac + lacI q pro ] (DE3) gor522 ::Tn 10 trxB Str r Tet r } (bioNova Científica s.l.), and XL10-Gold {Tet r Δ( mcrA ) 183 Δ( mcrCB - hsdSMR-mrr ) 173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte F′[ pro AB lacI q Z ΔM15 Tn 10 (Tet r ) Kan r ]} (Stratagene).

Techniques: Activity Assay, Fluorescence, Produced

Journal: Applied and Environmental Microbiology

Article Title: Disulfide Bond Formation and Activation of Escherichia coli ?-Galactosidase under Oxidizing Conditions

doi: 10.1128/AEM.06923-11

Figure Lengend Snippet: Amounts of soluble protein observed by Western blotting. rβ-Galactosidase (A) and rGFP (B) were produced in the wild-type (wt [MC4100]) and Origami strains. The P values, obtained by a t test, are 0.056 (A) and 0.076 (B).

Article Snippet: The Escherichia coli strains used in this work were the K-12 derivatives MC4100 [ araD139 Δ( argF-lac ) U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR Str r ] ( 43 ), Origami 2 {Δ( ara-leu ) 7697 Δ lacX74 Δ phoA PvuII phoR araD139 ahpC galE galK rpsL F′[ lac + lacI q pro ] (DE3) gor522 ::Tn 10 trxB Str r Tet r } (bioNova Científica s.l.), and XL10-Gold {Tet r Δ( mcrA ) 183 Δ( mcrCB - hsdSMR-mrr ) 173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte F′[ pro AB lacI q Z ΔM15 Tn 10 (Tet r ) Kan r ]} (Stratagene).

Techniques: Western Blot, Produced

Journal: Applied and Environmental Microbiology

Article Title: Disulfide Bond Formation and Activation of Escherichia coli ?-Galactosidase under Oxidizing Conditions

doi: 10.1128/AEM.06923-11

Figure Lengend Snippet: Nondenaturing polyacrylamide electrophoresis gel of rβ-galactosidase produced in the wild-type (wt [MC4100]) and Origami strains. Gels were analyzed in the absence (A) or presence (B) of the reducing agent DTT. R1, R2, and R3 correspond to three Origami replicas, while R4, R5, and R6 correspond to three replicas from the wild-type strain. C+ corresponds to a commercial E. coli β-galactosidase. Arrows indicate the positions of the electrophoretic bands.

Article Snippet: The Escherichia coli strains used in this work were the K-12 derivatives MC4100 [ araD139 Δ( argF-lac ) U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR Str r ] ( 43 ), Origami 2 {Δ( ara-leu ) 7697 Δ lacX74 Δ phoA PvuII phoR araD139 ahpC galE galK rpsL F′[ lac + lacI q pro ] (DE3) gor522 ::Tn 10 trxB Str r Tet r } (bioNova Científica s.l.), and XL10-Gold {Tet r Δ( mcrA ) 183 Δ( mcrCB - hsdSMR-mrr ) 173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte F′[ pro AB lacI q Z ΔM15 Tn 10 (Tet r ) Kan r ]} (Stratagene).

Techniques: Electrophoresis, Produced

Journal: Applied and Environmental Microbiology

Article Title: Disulfide Bond Formation and Activation of Escherichia coli ?-Galactosidase under Oxidizing Conditions

doi: 10.1128/AEM.06923-11

Figure Lengend Snippet: Specific enzymatic activities of rβ-galactosidase produced in the wild-type (wt [MC4100]) and Origami strains. Specific activity was determined without DTT (filled bars) and with increasing concentrations of DTT: 10 mM (light shaded bars) and 20 mM (dark shaded bars). The P values, obtained by a t test, are 0.028 for the wt strain and 0.043 for the Origami strain.

Article Snippet: The Escherichia coli strains used in this work were the K-12 derivatives MC4100 [ araD139 Δ( argF-lac ) U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR Str r ] ( 43 ), Origami 2 {Δ( ara-leu ) 7697 Δ lacX74 Δ phoA PvuII phoR araD139 ahpC galE galK rpsL F′[ lac + lacI q pro ] (DE3) gor522 ::Tn 10 trxB Str r Tet r } (bioNova Científica s.l.), and XL10-Gold {Tet r Δ( mcrA ) 183 Δ( mcrCB - hsdSMR-mrr ) 173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte F′[ pro AB lacI q Z ΔM15 Tn 10 (Tet r ) Kan r ]} (Stratagene).

Techniques: Produced, Activity Assay

Journal: Applied and Environmental Microbiology

Article Title: Disulfide Bond Formation and Activation of Escherichia coli ?-Galactosidase under Oxidizing Conditions

doi: 10.1128/AEM.06923-11

Figure Lengend Snippet: Specific activities of rβ-galactosidase (filled bars) and rβ-galactosidase C500A (open bars). rβ-Galactosidase activities (expressed as the percentage of β-galactosidase activity in rβ-galactosidase C500A with respect to the activity of wild-type rβ-galactosidase) were determined in both the wild-type (wt [MC4100]) and Origami E. coli strains. The P values, obtained by a t test, are 0.086 for the wt and 0.040 for the Origami strain.

Article Snippet: The Escherichia coli strains used in this work were the K-12 derivatives MC4100 [ araD139 Δ( argF-lac ) U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR Str r ] ( 43 ), Origami 2 {Δ( ara-leu ) 7697 Δ lacX74 Δ phoA PvuII phoR araD139 ahpC galE galK rpsL F′[ lac + lacI q pro ] (DE3) gor522 ::Tn 10 trxB Str r Tet r } (bioNova Científica s.l.), and XL10-Gold {Tet r Δ( mcrA ) 183 Δ( mcrCB - hsdSMR-mrr ) 173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte F′[ pro AB lacI q Z ΔM15 Tn 10 (Tet r ) Kan r ]} (Stratagene).

Techniques: Activity Assay

Journal:

Article Title: Functional Analysis of PvdS, an Iron Starvation Sigma Factor of Pseudomonas aeruginosa

doi:

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: Antibiotics were used in selective media at the following concentrations: tetracycline, 12.5 μg/ml for E. coli and 100 μg/ml for P. aeruginosa ; chloramphenicol, 30 μg/ml for E. coli and 100 μg/ml for P. aeruginosa ; kanamycin, 25 μg/ml for E. coli , 50 μg/ml for P. putida , and 300 μg/ml for P. aeruginosa ; and ampicillin, (100 μg/ml), nalidixic acid (20 μg/ml), and streptomycin (25 μg/ml) for E. coli . table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Genotype and/or relevant characteristics Reference or source E. coli DH5αF′ recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 Δ( lacZYA-argF ) U169 [Φ80d lacZ ΔM15] Nal r 44 M15 lac1 q ara-14 galK2 mtl-1 F − 49 MC4100 araD139 rpsL150 relA1 flbB5301 deoC1 pstF25 rbsR Δ( lacZYA-argF ) U169 Str r 5 P. aeruginosa PAO1 (ATCC 15692) Prototroph American Type Culture Collection 8830 his-1 9 8830R2::Cm his-1 algR2 ::Cm 42 P. putida WCS358 Prototroph 14 WCS358.E9 pfrA ::Tn 3 Gus 52 VM119 pfrI ::Tn 5 51 Plasmids pQE60 Expression vector; ColE1 replicon, P T5 lacO RBSII Ap r Diagen pDMI,1 Repressor plasmid; p15A replicon, lac1 q Km r 6 pRK2013 Helper plasmid; ColE1 replicon, Km r Mob + Tra + 12 pMP190 Broad-host-range, low-copy-number promoter probe vector; IncQ replicon, lacZ Cm r Tra − 47 pMP220 Broad-host-range, low-copy-number promoter probe vector; IncP replicon, lacZ Tc r Tra − 47 pPV51 Promoter of pvdA pyoverdin synthesis gene cloned into pMP220 20 pMP190::P pvdD Promoter of pvdD pyoverdin synthesis gene cloned into pMP190 8 pMP190::P pvdE Promoter of pvdE pyoverdin synthesis gene cloned into pMP190 8 pBRXB 1.8-kb Xho I- Bam HI fragment containing pvdS ligated to pBR322 8 pPvdS6H The 564-bp pvdS coding sequence ligated to the Nco I- Bgl II sites of pQE60 in frame to the His 6 coding sequence This study pPvdSF The 564-bp pvdS coding sequence ligated to the Nco I- Hin dIII sites of pQE60 in frame to the FLAG coding sequence This study pPvdSWT The 564-bp pvdS coding sequence ligated to the Nco I- Hin dIII sites of pQE60 This study Open in a separate window Bacterial strains and plasmids used in this study

Techniques: Plasmid Preparation, Expressing, Clone Assay, Sequencing

Journal: BioTechniques

Article Title: A robust fractionation method for protein subcellular localization studies in Escherichia coli.

doi: 10.2144/btn-2018-0135

Figure Lengend Snippet: Figure 1. Evaluation of periplasmic and cytoplasmic extraction methods. W3110 WT and MC4100 Tat-null strains co-expressed cytoplasmic His-tagged PhoA and C-terminally cMyc-tagged Erv1p and PDI proteins. The cells were fractionated according to methods 1 (cold osmotic shock and freeze– thaw cycling), 2 (cold osmotic shock with magnesium and freeze–thaw cycling), 3 (EDTA/lysozyme/ cold osmotic shock and sonication) and 4 (EDTA/lysozyme/cold osmotic shock and freeze–thaw cycling). The fractions are represented as cytoplasmic (C), insoluble (I) and periplasmic (P). Western blots show from top to bottom, the detection of PhoA, PDI, Erv1p, G6PD, LacI, MBP and DsbA proteins as detected by anti-His, anti-cMyc, anti-cMyc, anti-G6PD, anti-LacI, anti-MBP and anti-DsbA antibodies, respectively. Molecular weight markers are indicated on the right in kDa. WT: Wildtype.

Article Snippet: Strain Phenotype Genotype Origin W3110 Wildtype F-, lambda-, IN(rrnD-rrnE)1, rph-1 ATCC 27325 MC4100 Wildtype F-, [araD139]B/r, Del(argF-lac)169, lambda-, e14-, flhD5301, Δ(fruK-yeiR)725(fruA25), relA1, rpsL150(strR), rbsR22, Del(fimB-fimE)632(::IS1), deoC1 CGSC 6152 MC4100 Tat-null F-, [araD139]B/r, Del(argF-lac)169, lambda-, e14-, flhD5301, Δ(fruK-yeiR)725(fruA25), relA1, rpsL150(strR), rbsR22, Del(fimB-fimE)632(::IS1), deoC1, ΔtatABCDE Ref [34] btn-2018-0135.indd 172 10/04/2019 12:04 www.BioTechniques.com No. 4 | Vol.

Techniques: Extraction, Sonication, Western Blot, Molecular Weight

Journal: BioTechniques

Article Title: A robust fractionation method for protein subcellular localization studies in Escherichia coli.

doi: 10.2144/btn-2018-0135

Figure Lengend Snippet: Figure 2. Evaluation of optimized subcellular fractionation method. W3110 WT and MC4100 Tat-null strains co-expressing periplasmic (Tat pathway specific) His-tagged TorASP-PhoA and C-termi- nally cMyc-tagged Erv1p and PDI were grown in different conditions: induced in LB (1) for 2 h at 30°C or (2) for 4 h at 30°C, (3) for 2 h at 37°C, (4) for 16 h at 18°C or (5) induced in 2x TY for 2 h at 30°C or (6) induced in fed-batch culture for 16 h at 18°C. The cells were then fractionated using PureFrac method into the cytoplasmic (C), insoluble (I) and periplasmic (P) fractions. (A) Western blots of PhoA-His detected by anti-His antibodies. (B) Western blot showing Erv1p, PDI, LacI and MBP proteins detected by anti-cMyc, anti-cMyc, anti-LacI and anti-MBP antibodies respectively. Molecular weight markers are indicated in kDa on the right of each blot. WT: Wildtype.

Article Snippet: Strain Phenotype Genotype Origin W3110 Wildtype F-, lambda-, IN(rrnD-rrnE)1, rph-1 ATCC 27325 MC4100 Wildtype F-, [araD139]B/r, Del(argF-lac)169, lambda-, e14-, flhD5301, Δ(fruK-yeiR)725(fruA25), relA1, rpsL150(strR), rbsR22, Del(fimB-fimE)632(::IS1), deoC1 CGSC 6152 MC4100 Tat-null F-, [araD139]B/r, Del(argF-lac)169, lambda-, e14-, flhD5301, Δ(fruK-yeiR)725(fruA25), relA1, rpsL150(strR), rbsR22, Del(fimB-fimE)632(::IS1), deoC1, ΔtatABCDE Ref [34] btn-2018-0135.indd 172 10/04/2019 12:04 www.BioTechniques.com No. 4 | Vol.

Techniques: Fractionation, Expressing, Western Blot, Molecular Weight

Journal: BioTechniques

Article Title: A robust fractionation method for protein subcellular localization studies in Escherichia coli.

doi: 10.2144/btn-2018-0135

Figure Lengend Snippet: Figure 3. Validation of PureFrac. An empty vector (1), cytoplasmic His-tagged PhoA (2), periplasmic (Sec pathway) OmpASP-PhoA (3) and periplasmic (Tat pathway) TorASP-PhoA co-expressed with cMyc-tagged PDI and Erv1p (4) were expressed in MC4100 WT and Tat-null strains. The cells were then fractionated using PureFrac method into the cytoplasmic (C), insoluble (I) and periplasmic (P) fractions. Western blots show PhoA-His, Erv1p, PDI, LacI and MBP proteins detected by anti-His, anti-cMyc, anti-cMyc, anti-LacI and anti-MBP antibodies, respectively. Molecular weight markers are indicated on the right in kDa. WT: Wild type.

Article Snippet: Strain Phenotype Genotype Origin W3110 Wildtype F-, lambda-, IN(rrnD-rrnE)1, rph-1 ATCC 27325 MC4100 Wildtype F-, [araD139]B/r, Del(argF-lac)169, lambda-, e14-, flhD5301, Δ(fruK-yeiR)725(fruA25), relA1, rpsL150(strR), rbsR22, Del(fimB-fimE)632(::IS1), deoC1 CGSC 6152 MC4100 Tat-null F-, [araD139]B/r, Del(argF-lac)169, lambda-, e14-, flhD5301, Δ(fruK-yeiR)725(fruA25), relA1, rpsL150(strR), rbsR22, Del(fimB-fimE)632(::IS1), deoC1, ΔtatABCDE Ref [34] btn-2018-0135.indd 172 10/04/2019 12:04 www.BioTechniques.com No. 4 | Vol.

Techniques: Biomarker Discovery, Plasmid Preparation, Western Blot, Molecular Weight

Journal: BMC Complementary and Alternative Medicine

Article Title: Antibacterial activities of the methanol extracts and compounds from Erythrina sigmoidea against Gram-negative multi-drug resistant phenotypes

doi: 10.1186/s12906-015-0978-8

Figure Lengend Snippet: Bacterial strains used and their features

Article Snippet: The studied microorganisms included sensitive and resistant strains of Escherichia coli (ATCC8739, AG100, AG100A, AG100A TET , AG102, MC4100, W3110), Enterobacter aerogenes (ATCC13048, CM64, EA27, EA289, EA294, EA298), Enterobacter cloacae (ECCI69, BM47, BM67), Klebsiella pneumoniae (ATCC12296, KP55, KP63, K24, K2), Providencia stuartii (NEA16, ATCC29916, PS2636, PS299645) and Pseudemonas aeruginosa (PA01, PA124) obtained clinically or from the American Type Culture Collection.

Techniques: Mutagenesis, Variant Assay

Journal: BMC Complementary and Alternative Medicine

Article Title: Antibacterial activities of the methanol extracts and compounds from Erythrina sigmoidea against Gram-negative multi-drug resistant phenotypes

doi: 10.1186/s12906-015-0978-8

Figure Lengend Snippet: MICs and MBC (μg/mL) of the crude extracts from Erythrina sigmoidea and chlroramphenicol on the panel of tested bacteria

Article Snippet: The studied microorganisms included sensitive and resistant strains of Escherichia coli (ATCC8739, AG100, AG100A, AG100A TET , AG102, MC4100, W3110), Enterobacter aerogenes (ATCC13048, CM64, EA27, EA289, EA294, EA298), Enterobacter cloacae (ECCI69, BM47, BM67), Klebsiella pneumoniae (ATCC12296, KP55, KP63, K24, K2), Providencia stuartii (NEA16, ATCC29916, PS2636, PS299645) and Pseudemonas aeruginosa (PA01, PA124) obtained clinically or from the American Type Culture Collection.

Techniques:

Journal: BMC Complementary and Alternative Medicine

Article Title: Antibacterial activities of the methanol extracts and compounds from Erythrina sigmoidea against Gram-negative multi-drug resistant phenotypes

doi: 10.1186/s12906-015-0978-8

Figure Lengend Snippet: MICs and MBC of compounds from the bark of Erythrina sigmoidea on selected bacteria

Article Snippet: The studied microorganisms included sensitive and resistant strains of Escherichia coli (ATCC8739, AG100, AG100A, AG100A TET , AG102, MC4100, W3110), Enterobacter aerogenes (ATCC13048, CM64, EA27, EA289, EA294, EA298), Enterobacter cloacae (ECCI69, BM47, BM67), Klebsiella pneumoniae (ATCC12296, KP55, KP63, K24, K2), Providencia stuartii (NEA16, ATCC29916, PS2636, PS299645) and Pseudemonas aeruginosa (PA01, PA124) obtained clinically or from the American Type Culture Collection.

Techniques:

Journal: Microbiological research

Article Title: Regulation of metE + mRNA expression by FnrS small RNA in Salmonella enterica serovar Typhimurium.

doi: 10.1016/j.micres.2019.126319

Figure Lengend Snippet: Fig. 1. The upstream sequences of the Salmonella Typhimurium LT2 metE+ gene and the FnrS sequences and its predicted secondary structure, using Mfold (http:://un- afold.rna.albany.edu). (A) The -35, -10 pro- moter and the SD sequence of the metE+ are marked by a filled line. The +1 is marked as the transcriptional start site and Met is the in- itial amino acid of the MetE protein. The metE::lacZ fusion plasmid includes that the metE+ promoter region is fused with the lacZ reporter gene by compatible EcoRI-BamHI sites. The black spots under the metE+ up- stream sequences are the putative com- plementary sequences for FnrS binding. (B) The nucleotide from 5′ end till position 46 is a stem-loop 1, the nucleotide 62 to 87 is a stem- loop 2, the nucleotide 95 to 114 is a stem-loop 3. The black dots indicate that the nucleotides of FnrS likely base-paired with the sequences of the metE+ 5′ mRNA.

Article Snippet: Strains Description Source or reference Salmonella enterica serovar strain Typhimurium LT2 Wild-type ATCC 700720 Escherichia coli MC4100 F-araD139D(argF-lac)U169 rpsL150 relA1 flhD5301 deoC1 fruA25 rbsR22 C600 F-e14- mcrA1 thr-1 thiE-1 thr-1 leuB6 lacY1 fhuA21 glnX44 rfbD1 XL1-blue supE44 hsdR17 recA1 endA1 gyrA96 thi relA1 lac- [F’ proAB+ lacIq lacZ △M15 Tn10 (terR)] BW25113 △lacZ4787(::rrnB-3) hsdR514 D(araD-araB)567D(rhaD-rhaB)568 rph-1 Barry L. Wanner JW3805 BW25113 DmetE::kan (Baba et al., 2006) JW1328 BW25113 Dfnr::kan (Baba et al., 2006) JW4130 BW25113 Dhfq::kan (Baba et al., 2006) DJ480 DlacX74 (lambda-based lacZ fusions) Ding Jin (NCI) YF 10001 MC4100 DmetE This study YF 10002 MC4100 DmetE Dhfq::FTR This study YF 10003 MC4100 DmetE DfnrS::FTR This study CW10002 DJ480 λmetE::lacZ (pr) This study CW10003 DJ480 DfnrS::kan λmetE::lacZ (pr) This study CW10004 DJ480 λmetE::lacZ (op) This study CW10005 DJ480 DfnrS::kan λmetE::lacZ (op) This study CW10006 DJ480 DmetE::kan λmetE::lacZ (pr) This study CW10007 DJ480 DmetE::FRT DfnrS::kan λmetE::lacZ (pr) This study CW10008 DJ480 DmetE::FRTDfnr::kan λmetE::lacZ (pr) This study CW10009 DJ480DmetE::FRT DfnrS::FRT Dfnr::kan λmetE::lacZ (pr) This study CW10014 DJ480 DfnrS::kan λmetE2::lacZ (op) This study CW10015 DJ480 DfnrS::kan λmetE54::lacZ (op) This study Plasmids pBAD33 ori (pACYC18) araC PBAD cmr (Guzman et al., 1995) pBR-PLAC ori (pMB1) PLAC promoter based expression vector ampr (Guillier et al., 2006) pRS414 bla-Tl4 EcoR I-Sma I-BamH I lacZ (pr) (Simons et al., 1987) pRS415 bla-Tl4 EcoR I-Sma I-BamH I lacZ (op) (Simons et al., 1987) pCP20 FLP+ lcI857+ lPR Repts ampr cmr (Cherepanov and Wackernagel, 1995) pYF11 pBAD33-184bp 5’-UTR-metE +-ha This study pYF16 pRS414-PmetE::lacZ fusion gene This study pYF17 pRS415-PmetE::lacZ fusion gene This study pYF18 pBR-PLAC-fnrS+ This study pYF19 pBR-PLAC-fnrS Dloop 2 This study pYF20 pBR-PLAC-fnrS Dloop 2∼3 This study pCW3 pBR-PLAC-fnrS I (U57A/U58G/U59A) This study pCW4 pBR-PLAC-fnrS II (C47A/U48A/U49G) This study pCW5 pBR-PLAC-fnrS III (G4C/G5T) This study pCW6 pBR-PLAC-fnrS 41 (U41G) This study pCW7 pBR-PLAC-fnrS 43 (U43G) This study pCW8 pBR-PLAC-fnrS 54 (C54G) This study Phages lRS45 imm21 ind+ bla'-lacZSC (Simons et al., 1987) P1via lmetE::lacZ (op) carrying metE+ promoter with lacZ+ This study lmetE::lacZ (pr) carrying metE+ promoter with ‘lacZ This study lmetE-2::lacZ (op) (A-2T)(A-3C) relative to (+1) ATG This study lmetE54::lacZ(op) (G-8C) relative to (+1) ATG This study

Techniques: Sequencing, Plasmid Preparation, Binding Assay

Journal: Journal of the American Chemical Society

Article Title: Bioinformatic expansion and discovery of thiopeptide antibiotics

doi: 10.1021/jacs.8b03896

Figure Lengend Snippet: Antibiotic activity of saalfelduracin and thiostrepton as determined by microbroth dilution assay. MIC, minimum inhibitory concentration.

Article Snippet: Sterne 0.25 0.25 Escherichia coli MC4100 >64 >64 Pseudomonas aeruginosa PAO1 >64 >64 Acinetobacter baumannii ATCC 19606 >64 >64 Open in a separate window Antibiotic activity of saalfelduracin and thiostrepton as determined by microbroth dilution assay.

Techniques: Activity Assay, Dilution Assay, Concentration Assay